Gentaur's Products: Quality diagnostic kits, Enzymatic Assays, ELISA Kits, One-Step Rapid Tests and Chemistry Reagents

For the quantitative colorimetric determination of AST (SGOT) in serum. Serum aspartate aminotransferase (AST), also known as serum glutamic oxalacetic transaminase (SGOT), is a tissue enzyme that catalyzes the exchange of amino and keto groups between alpha amino acids and alpha-keto acids. AST is widely distributed in tissue principally cardiac, hepatic, muscle and kidney. Injury to these tissues results in the release of the AST (SGOT) enzyme to general circulation. Following a myocardial infarction, serum levels of AST (SGOT) are elevated and reach a peak 48 to 60 hours after onset. Hepatobiliary diseases, such as cirrhosis, metastatic carcinoma, and viral hepatitis also will increase serum AST levels. Methods for the determination of serum AST (SGOT) include ultraviolet kinetic analysis and colorimetric methods. Earlier colorimetric methods were based on the reaction of oxalacetate with dinitrophenylhydrazine. However, this reaction is time consuming and non-specific reactions. The present method is based on a modification of the colorimetric method by Doumas and Briggs, which offers increased specificity and shortened incubation time.